Highly controllable oligonucleotide-nanomaterials interface for next-generation biological detection

PROJECT COST:

1 285 668 $

PRIMA CONTRIBUTION:

583 668 $

PROJECT DETAILS:

Nanomaterials have advanced applications in the life sciences. In particular, the magnetic nanoparticles (MNPs) and the gold nanoparticles (AuNPs) are being used as promising candidates for biosensing applications, similar to rapid tests for COVID-19 and electrochemical applications, including via DNA molecule designs. Next-generation nanomaterials are generating interest for the manipulation of DNA (and RNA). In fact, some advanced DNA sequencing technologies use DNA-wrapped beads. This illustrates the importance of the interface between nanomaterials and DNA oligonucleotides.

The project proposed by Professor Jonathan Perreault of INRS-Institut Armand-Frappier, in collaboration with other INRS professors and the company Galenvs Sciences, has two objectives/deliverables: 1) To develop amidite bead “building blocks” for integration into synthetic DNA oligonucleotides, useful for high-throughput purification and selection (and various types of molecular biology experiments); 2) To develop MNPs as solid substrates for repetitive polymerization to produce oligopolymers for different applications, which may (or may not) include analogous nucleotides to add chemical functionality.

Two expected results: 1) A new class of nanomaterials, synthesizable at high capacity based on a controlled MNP/oligonucleotide interaction, 2) New techniques for oligonucleotide purification.

The development of these technologies will enable Québec companies, such as Galenvs Sciences, to offer DNA oligonucleotide synthesis and high-throughput sequencing projects and services for the diagnostics industry and other organizations involved in developing diagnostic platforms or tests. Galenvs Sciences will be a key partner in implementing a value chain in the life sciences sector in Québec. This project will provide training for two postdoctoral fellows, seven master’s students, six doctoral students, and twelve interns.